However, IC50 calculation tutorials could be improved adding real life examples with common absorbance or fluorescence experiments. GraphPad tutorial is good and very informative. Figures are amazing and you can easily add standard deviation, which is a must to in scientific publications. I've tried other software before and GraphPad is way easier to use and complete. It's pretty easy to import data and replicates and perform analysis over these replicates. That's an incredible advantage for people who's learning and selecting their "go-to" statistic software.
GraphPad allows you to have one month free trial with all the features. It's an amazing software and it allowed me to transition from Excel and Origin and only use GraphPad now. I've also generated my charts, figures and reports directly to publish in peer-reviewed journals because of their quality. Comentarios: I have used GraphPad to calculate IC50, EC50 in fluorescence-based assays for my scientific project.
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